Nano Flow Cytometry: Detection, Quantification and sorting of Extracellular Vesicles and Viruses
Extracellular vesicles (EVs), small vesicles that are released by virtually all cell types, with an innate ability to mediate the transmission of signaling molecules (proteins, small RNAs, and DNA) between cells are among the factors that are involved in the communication between cells.
Compared to conventional flow cytometry, Nano cytometry is an emerging and highly sensitive approach for the characterization of Extracellular Vesicles (EVs) and Viral particules.
Interestingly, labeling of the particules with a fluorescent dye is a NOT prerequisite step for detection, size determination and quantification. In this study, we aimed to evaluate the efficiency of the Nano AnalyzerTM for the detection of subcellular objects based on SSC sensitive signal and a specific discrimination from the background noise with or without fluorescence expression in several vesicules types.
Our work shows that NanoCytometry is as a method of choice for single EVs and virions analysis, and the NanoFCM allows the most rapid and effective method to quantified and size measurement of small particles. We are now investigating whether the preanalytical step can affected the reliability of the results by flow cytometry analysis.